Unveiling the mechanisms of breast cancer pathogenesis by proteomics methods

Patogenezu složene i heterogene bolesti kao što je to rak dojke nije moguće u potpunosti razjasniti klasičnim metodološkim pristupima koji su se koristi li posljednjih desetljeća u molekularnoj medicini. Osobito je važno otkriti nove biljege koji bi pomogli u ranoj detekciji bolesti te služili za razvoj ciljane terapije. Dosadašnji rezultati iz područja istraživanja raka dojke u kombinaciji s globalnim metodama istraživanja statusa ekspresije gena i proteina pokazali su kako je u patogenezu raka dojke uključen ljudski hormon rasta (hGH) te mehanizmi autokrine regulacije preko proteina Pax-5. Također je identi fi ciran čitav niz novih potencijalnih biomarkera kao što su to HOXA1, CHOP SH3GLB1, kazein kinaze, p53, aneksin XI, CDC25C, eIF- 4E i MAP kinaze 7, 14-3-3e, galekti n-1, aneksin-5, aneksin-1, LDH-B, GST-pi, akti n, vimenti n, HSP70, CK18, moezin, SH3GLB1, SUB1, SND1 i TRIM28, osteoponti n i osteonekti nBreast cancer is a complex and heterogenic disease. Classical molecular medical approaches cannot be successfully used to completely understand its pathogenesis. In additi on, fi nding new biological markers that would help in early detecti on and creati on of guided and specifi c therapy is crucial for the future breast cancer management. The results of breast cancer research in combinati on with those obtained by large scale methods that examine the expression status of genes and proteins, have pointed to an important molecule, namely the human growth hormone (hGH), as a major player in the pathogenesis of breast cancer. It acts through the autocrine regulati on system where the acti vati on of Pax-5 gene has been recently confi rmed as well. A large number of new biomarkers, like HOXA1, CHOP SH3GLB1, casein kinase, p53, annexin XI, CDC25C, eIF-4E, MAP kinase 7, 14-3-3e, galecti n-1, annexin-5, annexin-1, LDH-B, GST-pi, acti n, vimenti n, HSP70, CK18, moesin, SH3GLB1, SUB1, SND1 i TRIM28, osteoponti n and osteonecti n, have been recently identi fi ed as well