[[abstract]]Although several cell-based reporter assays have been developed for screening of viral protease inhibitors, most of these assays have a significant limitation in that numerous false positives can be generated for the compounds that are interfering with reporter gene detection due to the cellular viability. To improve, we developed a mammalian cell-based assay based on the reverse two-hybrid system to monitor the proteolytic activity of human enterovirus 71 (EV71) 3C protease and to validate the cytotoxicity of compounds at the same time. In this system, the GAL4 DNA binding domain (M3) and transactivation domain (VP16) were fused, in-frame, with 3C or 3C(mut). The 3C(mut) was an inactivated protease with mutations at the predict...
Hepatitis C virus (HCV) is a major pathogen of community-acquired and post-transfusional non-A, non-...
Enterovirus 71 (EV71), a member of Picornaviridae, is one of the major pathogens of human hand, foot...
Over the years, the view on proteases as relatively non-specific protein degradation enzymes, mainly...
[[abstract]]Although several cell-based reporter assays have been developed for screening of viral p...
Proteolytical cleavage of the picornaviral polyprotein is essential for viral replication. Therefore...
<p>Proteolytical cleavage of the picornaviral polyprotein is essential for viral replication. ...
[[abstract]]The hepatitis C virus (HCV) contains a positive-sense RNA genome that encodes a unique p...
[[abstract]]Human enterovirus (EV) belongs to the picornavirus family, which consists of over 200 me...
Objectives: Human enterovirus 71 (EV-71), a member of the Enterovirus genus, constitutes a major pub...
[[abstract]]The 3C protease (3C(pro)) of enterovirus 71 (EV71) is a good molecular target for drug d...
[[abstract]]A cell culture system for the production of hepatitis C virus (HCV) whole virions has gr...
[[abstract]]Hepatitis C virus ( HCV) encodes a viral protease, nonstructural (NS) 3/4A, that is crit...
We have determined the cleavage specificity and the crystal structure of the 3C protease of enterovi...
Identification of novel cellular proteins as substrates to viral proteases would provide a new insig...
[[abstract]]Hepatitis C virus (HCV) encodes a polyprotein that needs to be processed proteolytically...
Hepatitis C virus (HCV) is a major pathogen of community-acquired and post-transfusional non-A, non-...
Enterovirus 71 (EV71), a member of Picornaviridae, is one of the major pathogens of human hand, foot...
Over the years, the view on proteases as relatively non-specific protein degradation enzymes, mainly...
[[abstract]]Although several cell-based reporter assays have been developed for screening of viral p...
Proteolytical cleavage of the picornaviral polyprotein is essential for viral replication. Therefore...
<p>Proteolytical cleavage of the picornaviral polyprotein is essential for viral replication. ...
[[abstract]]The hepatitis C virus (HCV) contains a positive-sense RNA genome that encodes a unique p...
[[abstract]]Human enterovirus (EV) belongs to the picornavirus family, which consists of over 200 me...
Objectives: Human enterovirus 71 (EV-71), a member of the Enterovirus genus, constitutes a major pub...
[[abstract]]The 3C protease (3C(pro)) of enterovirus 71 (EV71) is a good molecular target for drug d...
[[abstract]]A cell culture system for the production of hepatitis C virus (HCV) whole virions has gr...
[[abstract]]Hepatitis C virus ( HCV) encodes a viral protease, nonstructural (NS) 3/4A, that is crit...
We have determined the cleavage specificity and the crystal structure of the 3C protease of enterovi...
Identification of novel cellular proteins as substrates to viral proteases would provide a new insig...
[[abstract]]Hepatitis C virus (HCV) encodes a polyprotein that needs to be processed proteolytically...
Hepatitis C virus (HCV) is a major pathogen of community-acquired and post-transfusional non-A, non-...
Enterovirus 71 (EV71), a member of Picornaviridae, is one of the major pathogens of human hand, foot...
Over the years, the view on proteases as relatively non-specific protein degradation enzymes, mainly...