Molecular Diagnostics of Sexually Transmitted Infections

Metode molekularne dijagnostike dio su rutinskoga dijagnostičkog algoritma spolno prenosivih infekcija. Molekularna dijagnostika spolno prenosivih infekcija u kliničkim laboratorijima uglavnom se temelji na primjeni standardiziranih amplifi kacijskih molekularnih testova. Najčešće se rabe metode lančane reakcije polimerazom (polymerase chain reaction, PCR), PCR u realnom vremenu (real-time PCR), tekućinska hibridizacija (hybrid capture), amplifi kacija posredovana transkripcijom (transcription-mediated amplifi cation) i tehnologija izmjene lanaca (strand displacement technology). Primjenom ovih metoda možemo detektirati uzročnike spolno prenosivih infekcija u različitim biološkim uzorcima, tj. obriscima cerviksa, vagine, uretre i u urinu.Molecular methods are a part of the routine diagnostic algorithm for sexually transmitted infections. Molecular diagnosis of sexually transmitted infections in routine clinical laboratories is based on standardized amplifi cation assays. The majority of assays are based on polymerase chain reactions (PCR), real-time PCR, soluble hybridization, transcription- -mediated amplifi cation, and strand displacement technology. Molecular methods enable us to test a variety of biological samples including cervical, vaginal, and urethral swabs, as well as urine