Adherence of Candida Albicans to Different Finished Surfaces of PMMA Resins Reinforced with Glass E Fibers

Candida albicans has been widely associated with the etiology of denture-related stomatitis. When fiber reinforcement is used in the denture base as a total fiber reinforcement, the fibers may be exposed during the finishing of the denture. This may lead to oral disease if the fibers come into contact with the oral mucosa. In this study the adherence of candida albicans to the surface of PMMA and to the surface of E glass fibers of composite material used in dentures was compared. E glass fibers coated with Silan 1(silan + epoxy resin) and Silan 2 (silan + polyester resin) were used to reinforce denture base polymethyl metacrylate resin. Specimens were produced by two different methods. In the first group the fibers were wetted with a mixture of polymer powder and monomer liquid. In the second group the fibers were wetted in polymer liquid for 15 minutes and than blended with PMA polymers. All of the samples were heat cured. Half the prepared specimens were polished and the other half left unpolished. Replicates and yeast cells (Candida albicans ATCC, 90028, 1.0 x 107 cells/ml)were placed in wells and incubated for 1 h at 37°C on an orbital shaker at 80 rpm. The replicates were air dried and mounted on glass slides and stained with a solution of 0.5% crystal violet and 1.0% iodine. The number of adherant yeast cells were counted under a light microscope (400x). RESULTS: 1. Less Candida albicans adhered to the Polished surface (P< 0.01). 2. The highest Candida albicans accumulation was observed on the Silan 2(silan + poly ester resin) coupling samples.(P