Nadzor influence u dvorišne peradi i ptica selica u Sloveniji

Blood samples and clocal swabs were taken from free-living migratory birds and backyard flock birds. Enzyme linked immunosorbent assays (ELISA) and haemagglutination inhibition test (HI) was used to screen free-living migratory birds and backyard flocks for antibodies against avian influenza viruses (AIV). Pools of cloacal swabs were used for the attempt of isolation of AIV. Samples were inoculated of 9 to 11 day-old chicken embryos. Amnioallantoic fluid were collected and analysed for haemagglutination activity. Pools of cloacal swabs were used for the detection of AIV by RT-PCR. Sera samples of four out of twenty three geese were positive on presence of specific antibodies against AIV. All attempts of virus isolation were negative. No AIV nucleic acid was detected by RT-PCR.Uzeti su uzorci krvi i obrisci nečisnica slobodnoživućih ptica selica i dvorišne peradi. Imunoenzimni test i inhibicija hemaglutinacije korišteni su kako bi se u slobodnoživućih ptica selica i dvorišne peradi utvrdila nazočnost protutijela za viruse influence ptica. Skupni uzorci briseva nečisnica korišteni su za izdvajanje virusa influence ptica. Uzorci su inokulirani u 9 do 11 dana stare kokošje embrije. Sakupljena je amnioalantoisna tekućina i pretražena na sposobnost hem-aglutinacije. Skupni uzorci brisova nečisnica korišteni su za dokaz virusa influence ptica pomoću RT-PCR. Četiri od 23 seruma gusaka bila su pozitivna na nazočnost specifičnih protutijela za virus influence ptica. Svi pokušaji izdvajanja virusa dali su negativan rezultat. Postupkom RT-PCR nije dokazana nazočnost nukleinske kiseline virusa influence ptica