Rheological and thermophysical properties of carragenan and β-lactoglobulin model systems treated with high hydrostatic pressure

Svrha ovog rada je ispitati utjecaj tretiranja visokim hidrostatskim tlakom na reološka i termofizička svojstva modelnih sustava β-laktoglobulina i karagenana. Suspenzije β-laktoglobulina tretirane su visokim hidrostatskim tlakom od 300 do 600 MPa. Vrijeme tretiranja bilo je 5 i 10 minuta. Mjerenja reoloških svojstava modelnih sustava karagenana i β-laktoglobulina provedena su rotacionim reometrom, Rheometric Scientific RM-180 (Rheometric Scientific, Inc., Piscataway, USA), na temperaturi od 20 ºC. Reološki parametri određeni su upotrebom Ostwald de Waele-ovog zakona. Rezultati ispitivanja pokazuju da su svi ispitivani sustavi imali ne-newtonski, pseudoplastični karakter, te da uzorci tretirani visokim hidrostatskim tlakom imaju promijenjene reološke značajke uslijed djelomične denaturacije proteina proporcionalne primijenjenom tlaku i vremenu tretiranja. Temperature faznih promjena određene su metodom diferencijalne termičke analize (DTA). Utvrđeno je da visoki hidrostatski tlak značajno snižava temperaturu zamrzavanja i odmrzavanja modelnih sustava β-laktoglobulina, te da karagenan posjeduje određena krioprotektorska svojstva.The aim of this paper is to examine the influence of high hydrostatic pressure treatment on the rheological and thermophisical properties of β-lactoglobulin and carragenan model systems. Suspensions of β-lactoglobulin were treated with a high hydrostatic pressure in a range of 300 to 600 MPa. Holding period was 5 and 10 minutes. Measurements were performed using rotational viscosimeter Rheometric Scientific RM-180 at 20 oC. The rheological parameters were determined by the Ostwald de Waele law. The results of the investigation have shown that all investigated systems are non-Newtonian – pseudoplastic. All samples treated with high hydrostatic pressure have changed rheological characteristics. The extent of protein denaturation was proportional to the intensity of applied pressure and holding time. The phase transition temperatures were determined by differential thermal analysis (DTA). High pressure treatment caused depression of freezing point and melting point, respectively. Carragenan acts as a crioprotectant