Utjecaj prehrane obogaćene n-6 i n-9 masnim kiselinama na ugradnju masnih kiselina u tkivne fosfolipide miša

The aim of this study was to investigate the phospholipid fatty acid profile and dietary fatty acids effects in the liver, lung, spleen and submandibular gland mice tissues. Palmitic (C16:0) and stearic (C18:0) fatty acids were major saturates in all the tissues studied. Oleic acid (C18:1n-9) was the major monounsaturated in the phospholipid fraction of all tissues. Docosahexaenoic acid (C22:6n-3) was the major polyunsaturated in the liver and lung, arachidonic acid (C20:4n-6) was the major polyunsaturated in the spleen, and linoleic acid (C18:2n-6) in the submandibular gland tissue samples. Presented data support the hypothesis that corn oil diet induced changes increase linoleic acid (C18:2n-6), and olive oil increases the oleic acid (C18:1n-9) content in total phospholipid fatty acid composition in the majority of the examined tissues. Liver tissue was most affected by the applied diet.Cilj je ovoga rada bio istražiti sastav masnih kiselina u fosfolipidima jetre, pluća, slezene i submandibularne žlijezde u miša te odrediti utjecaj prehranom unešenih masnih kiselina na sastav masnih kiselina ukupnih fosfolipida. U svim istraživanim tkivima najzastupljenije zasićene kiseline su palmitinska (C16:0) i stearinska (C18:0) kiselina, a oleinska kiselina (C18:1n-9) je najzastupljenija jednostrukonezasićena kiselina. Dokozaheksaenska kiselina (C22:6n-3) je najzastupljenija višestrukonezasićena kiselina u jetri i plućima, arahidonska kiselina (C20:4n-6) u slezeni, a linoleinska kiselina (C18:2n-6) u tkivu submandibularne žlijezde. Dobiveni rezultati podupiru hipotezu da je prehrana obogaćena kukuruznim uljem utjecala na povećanje linoleinske kiseline (C18:2n-6), a prehrana obogaćena maslinovim uljem na povećanje oleinske kiseline (C18:1n-9) u fosfolipidima istraživanih tkiva. Primjenjena prehrana najviše je utjecala na lipide tkiva jetre