Molecular techniques for diagnosis of animal deseases

Infectious laryngotracheitis (ILT) is an acute respiratory disease transmitted through respiratory secretions or fomites from naturally infected birds or the use of live vaccines. We investigated an outbreak of respiratory disease occurred in 2011 in Ecuador, a country where the disease was regarded as exotic. Viral detection was carried out by PCR (genes ICP4 and TK) and partial viral characterization was accomplished by amplicon sequencing. Our results suggested that the outbreak was caused by a field strain of ILTV