Application of BRET to monitor ligand binding to GPCRs
- Stoddart, Leigh A.
- Johnstone, Elizabeth K.M.
- Wheal, Amanda J.
- Goulding, Joelle
- Robers, Matthew B.
- Machleidt, Thomas
- Wood, Keith V.
- Hill, Stephen J.
- Pfleger, Kevin D.G.
DOIAbstract
Bioluminescence resonance energy transfer (BRET) is a well-established method for investigating protein-protein interactions. Here we present a BRET approach to monitor ligand binding to G protein–coupled receptors (GPCRs) on the surface of living cells made possible by the use of fluorescent ligands in combination with a bioluminescent protein (NanoLuc) that can be readily expressed on the N terminus of GPCRs
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