Tunable reagents for multi-functional bioconjugation: reversible or permanent chemical modification of proteins and peptides by control of maleimide hydrolysis
- Ryan, C.P.
- Smith, M.E.B.
- Schumacher, F.F.
- Grohmann, D.
- Papaioannou, D.
- Waksman, Gabriel
- Werner, F.
- Baker, J.R.
- Caddick, S.
DOIAbstract
Controlling maleimide hydrolysis allows the modular construction of bromomaleimide-mediated bioconjugates which are either stable or cleavable in an aqueous, thiol-mediated reducing environment. The application of this methodology to reversible protein biotinylation, the irreversible labeling of peptide disulfide bonds and the assembly of stable, fluorescein-labelled glycoprotein mimics is described
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