Evolutionary analysis of tomato Sw-5 resistance breaking isolates of Tomato spotted wilt virus

[EN] Tomato spotted wilt virus (TSWV) causes severe economic losses in many crops worldwide and often overcomes resistant cultivars used for disease control. Comparison of nucleotide and amino acid sequences suggested that tomato resistance conferred by the gene Sw-5 can be overcome by the amino acid substitution C to Y at position 118 (C118Y) or T120N in the TSWV movement protein, NSm. Phylogenetic analysis revealed that substitution C118Y has occurred independently three times in the studied isolates by convergent evolution, whereas the substitution T120N was a unique event. Analysis of rates of non-synonymous and synonymous changes at individual codons showed that substitution C118Y was positively selected. © 2011 SGM.This work was supported by the INIA (grants RTA04-004-C2 and RTA2008-00010-C03), the MEC (AGL2005-07754) and the Generalitat Valenciana (ACOMP/07/225 and ACOMP/2009/103). We would like to thank A. Barba for technical assistance and Professors Santiago F. Elena, Pedro Moreno and Bryce W. Falk for their excellent review of the manuscript and Daniel R. Pearce for English editing.López Del Rincón, C.; Aramburu, J.; Galipienso, L.; Soler Aleixandre, S.; Nuez Viñals, F.; Rubio, L. (2011). Evolutionary analysis of tomato Sw-5 resistance breaking isolates of Tomato spotted wilt virus. Journal of General Virology. 92:210-215. https://doi.org/10.1099/vir.0.026708-0S2102159