Optical detection of electrokinetically manipulated single molecules in a nanofluidic chip

We report on the progress of a novel nanofluidic device for detecting and manipulating single molecules in solution. This paper discusses the development of an earlier proposed molecule separation method, where electrokinetic forces separate different molecules based on their masses and charges. Optical imaging using confocal microscopy is applied to perform the detection of the single molecules. Potential applications of this device will be assessed. This research aims for the high spatial and spectral resolutions, both in manipulation and detection, which can lead to molecular identification.Quantitative Imaging GroupApplied Science