BIOTRANSFORMATIONS IN CARBOHYDRATE SYNTHESIS - N-ACETYLGALACTOSAMINYL AND N-ACETYLGLUCOSAMINYL TRANSFER ONTO METHYL ALPHA-GLUCOSIDES AND BETA-GLUCOSIDES CATALYZED BY THE BETA-N-ACETYLHEXOSAMINIDASE FROM ASPERGILLUS-ORYZAE

Regioselectivity of N-acetylhexosaminyl transfer onto methyl alpha- and beta-D-glucosides catalysed by the N-acetylhexosaminidase from Aspergillus oryzae is strongly dependent on the configuration at the anomeric centre of the acceptor: with the beta-glucoside, 1,3- and 1,4-transfer products are formed, whereas with the alpha-glucoside, 1,4- and 1,6-transfer products are obtained