Downstream effects of reduction in nucleotide excision repair in response to cisplatin treatment in melanoma

We have confirmed that the GGR regulators, BRCA1 and PCNA, are induced in normal cellular response to cisplatin induced DNA damage, but there is complete absence of induction of these regulators in melanoma cell lines. In addition, we identified highly significant overlap of transcript expression in melanoma cell lines after cisplatin treatment with transcripts highly correlated with the DNA doublestrand break repair genes BRCA1, BRCA2, ATM and CHEK2. Finally, we confirmed complete lack of induction of XPB in melanoma cell lines and a significant overlap of transcript expression with XPB deficient cells after UVR