One of the primary oxygen sensors in human cells, which controls gene expression by hydroxylating the hypoxia inducible transcription factor (HIFα) is the factor inhibiting HIF (FIH). As FIH is an alpha-ketoglutarate dependent non-heme iron dioxygenase, oxygen activation is thought to precede substrate hydroxylation. The coupling between oxygen activation and substrate hydroxylation was hypothesized to be very tight, in order for FIH to fulfill its function as a regulatory enzyme. Coupling was investigated by looking for reactive oxygen species production during turnover. Alkylsulfatase (AtsK), a metabolic bacterial enzyme with a related mechanism and similar turnover frequency, was used for comparison, and both FIH and AtsK were tested for...
Significance: Limited oxygen availability (hypoxia) commonly occurs in a range of physiological and ...
The ability of cells to sense and respond to sub-optimal levels of oxygen is a key requirement for o...
Prolyl Hydroxylase Domain 2 (PHD2) has been identified as a key oxygen sensor in humans along with F...
One of the primary oxygen sensors in human cells, which controls gene expression by hydroxylating th...
Oxygen is vital for aerobic life. A shortage of oxygen can produce disastrous outcomes to cellular f...
Oxygen is vital for aerobic life. A shortage of oxygen can produce disastrous outcomes to cellular f...
Factor Inhibiting HIF (FIH) is a Fe(II)-αKG dependent oxygenase that acts as a cellular oxygen senso...
Factor inhibiting HIF (FIH) is a cellular O<sub>2</sub>-sensing enzyme, which hydroxylates the hypox...
HIF (hypoxia-inducible factor) is an alphabeta transcription factor that modulates the hypoxic respo...
ABSTRACT: Factor inhibiting HIF (FIH) is a cellular O2-sensing enzyme, which hydroxylates the hypoxi...
Human hypoxia inducible factor (HIF) is responsible for mediating the body’s response to low O2 avai...
The Hypoxia Inducible Factors (HIFs) are widely expressed transcription factors critical for alterin...
Hypoxia Inducible Factor (HIF) is a transcription activator considered to be the main regulator of O...
The ability to adapt to variations in oxygen levels is essential for higher organisms. In animals, t...
The hypoxic response is a vast and complex system, delicately designed through evolution to allow ou...
Significance: Limited oxygen availability (hypoxia) commonly occurs in a range of physiological and ...
The ability of cells to sense and respond to sub-optimal levels of oxygen is a key requirement for o...
Prolyl Hydroxylase Domain 2 (PHD2) has been identified as a key oxygen sensor in humans along with F...
One of the primary oxygen sensors in human cells, which controls gene expression by hydroxylating th...
Oxygen is vital for aerobic life. A shortage of oxygen can produce disastrous outcomes to cellular f...
Oxygen is vital for aerobic life. A shortage of oxygen can produce disastrous outcomes to cellular f...
Factor Inhibiting HIF (FIH) is a Fe(II)-αKG dependent oxygenase that acts as a cellular oxygen senso...
Factor inhibiting HIF (FIH) is a cellular O<sub>2</sub>-sensing enzyme, which hydroxylates the hypox...
HIF (hypoxia-inducible factor) is an alphabeta transcription factor that modulates the hypoxic respo...
ABSTRACT: Factor inhibiting HIF (FIH) is a cellular O2-sensing enzyme, which hydroxylates the hypoxi...
Human hypoxia inducible factor (HIF) is responsible for mediating the body’s response to low O2 avai...
The Hypoxia Inducible Factors (HIFs) are widely expressed transcription factors critical for alterin...
Hypoxia Inducible Factor (HIF) is a transcription activator considered to be the main regulator of O...
The ability to adapt to variations in oxygen levels is essential for higher organisms. In animals, t...
The hypoxic response is a vast and complex system, delicately designed through evolution to allow ou...
Significance: Limited oxygen availability (hypoxia) commonly occurs in a range of physiological and ...
The ability of cells to sense and respond to sub-optimal levels of oxygen is a key requirement for o...
Prolyl Hydroxylase Domain 2 (PHD2) has been identified as a key oxygen sensor in humans along with F...