Various problems are encountered during DNA extraction from Vitis species, harbouring high levels of secondary metabolites and polysaccharides. A simple and highly efficient protocol for isolating large quantities (0.5±0.3 mg.g-1 of leaf tissue) of high-quality DNA, from dry young Vitis vinifera leaves, is described in the present study. Thus, three different DNA extraction protocols were examined. The isolated DNA is essentially free of polysaccharides, polyphenols, and other major contaminants as judged by viscosity, clear color, A260/280 ratio, and RAPD suitability. Moreover, the RAPD profiling from the isolated DNA was optimized to produce scorable and clear amplicons in all studied cultivars
A new and optimized protocol, here called 6hDNA (i.e. a genomic DNA obtained by a six-hour extracti...
Extraction of RNA from plant tissue containing high levels of polyphenols and polysaccharides is ted...
A DNA-based method has been applied to the identification of several musts and wines using microsate...
Use of a single seed is very useful for genetic studies on Vitis vinifera. However, molecular marker...
Molecular techniques and genetic studies require a fair amount of DNA of high quality in order to pr...
The genomic grapevine (Vitis vinifera L.) DNA extraction is difficult because of secondary metabolit...
Grapevine is a woody plant, whose high carbohydrate and phenolic compound contents usually interfere...
The extraction of DNA is a basic tool for molecular analyses, which is difficult for grapevine due t...
Aim: To compare different methods for extracting DNA from non-recalcitrant and recalcitrant tissues ...
The quality of wines is highly dependent on different factors among which the grapevine variety is o...
The need of accurate and reliable methods for DNA isolation and plant species identification in food...
WOS: 000339561100012The quality of wine depends on many factors. One of the most important is the se...
Reliable methods for DNA traceability in grapevines and wines is in great demand for protecting area...
Isolation of high quality RNA from plant tissues is one of the most critical steps for the successfu...
A new DNA extraction protocol has been established to reconstruct the genetic identity of grapevine ...
A new and optimized protocol, here called 6hDNA (i.e. a genomic DNA obtained by a six-hour extracti...
Extraction of RNA from plant tissue containing high levels of polyphenols and polysaccharides is ted...
A DNA-based method has been applied to the identification of several musts and wines using microsate...
Use of a single seed is very useful for genetic studies on Vitis vinifera. However, molecular marker...
Molecular techniques and genetic studies require a fair amount of DNA of high quality in order to pr...
The genomic grapevine (Vitis vinifera L.) DNA extraction is difficult because of secondary metabolit...
Grapevine is a woody plant, whose high carbohydrate and phenolic compound contents usually interfere...
The extraction of DNA is a basic tool for molecular analyses, which is difficult for grapevine due t...
Aim: To compare different methods for extracting DNA from non-recalcitrant and recalcitrant tissues ...
The quality of wines is highly dependent on different factors among which the grapevine variety is o...
The need of accurate and reliable methods for DNA isolation and plant species identification in food...
WOS: 000339561100012The quality of wine depends on many factors. One of the most important is the se...
Reliable methods for DNA traceability in grapevines and wines is in great demand for protecting area...
Isolation of high quality RNA from plant tissues is one of the most critical steps for the successfu...
A new DNA extraction protocol has been established to reconstruct the genetic identity of grapevine ...
A new and optimized protocol, here called 6hDNA (i.e. a genomic DNA obtained by a six-hour extracti...
Extraction of RNA from plant tissue containing high levels of polyphenols and polysaccharides is ted...
A DNA-based method has been applied to the identification of several musts and wines using microsate...