Fine-mapping and construction of a bovine contig spanning the ovine callipyge locus.
- Shay, T. L.
- Berghmans, S.
- SEGERS, Karin
- Meyers, S.
- Beever, J. E.
- Womack, J. E.
- Cockett, N. E.
- Georges, Michel
- Charlier, Carole
Publication date
January 2001
DOI Publisher
Springer Science and Business Media LLC ISSN
0938-8990 Citation count (estimate)
22Abstract
The callipyge (CLPG) gene was fine-mapped by linkage analysis to a 4.6-cM chromosome interval on distal ovine OAR18q, flanked by microsatellite markers IDVGA30 and OY3. The OAR18q linkage map and human HSA14q transcript map were aligned by genotyping two bovine-hamster whole-genome radiation hybrid panels with the microsatellite markers, as well as with sequences corresponding to HSA 14q genes. Using Type I loci mapping to the IDVGA30-OY3 interval as anchor points, we have constructed a 1.4-Mb bovine BAC contig containing the IDVGA30-OY3 interval. We demonstrate that the IDVGA30-OY3 interval spans approximately 770 kb and contains at least four genes: YY1, WARS, DLK1, and GTL2
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