Anti-proliferative Effects of Hydralazine on K562 Cell Line (CML)

Introduction: Cancer is the second leading cause of death in the world and among the types of cancer, leukemia is one of the deadliest. Chronic myeloid leukemia (CML) is the best known types of leukemia. Hydralazine is in the cardiovascular medical group, which is considering as inhibitory factor on the proliferation of cancer cells by knowing its inhibitory effects on DNA methyltransferase. The purpose of this study was to evaluate the inhibitory effects of hydralazine on K562 cells proliferation. Methods: First, the K562 cells were cultured. After, the different concentrations of the hydralazine were prepared and the cells were treated for 24, 48 and 72 hours. Next, the inhibitory effects of this drug on K562 cells proliferation was measured by MTT assay. Hoechst (33342) staining and DNA electrophoresis was used to check apoptosis. Data analyses were performed using SPSS software (Version 20) and ANOVA test (P