Eukaryotic cells have evolved a complex set of regulatory proteins to maintain genome stability by regulating DNA replication once and once per cell cycle. Despite intensive investigation, a precise understanding of the proteins that are phosphorylated during the initiation phase of DNA replication remains to be identified. Here we investigate the roles of cyclin E and cyclin A in the G1 to S-phase transition and reconstitute initiation of DNA replication in cyclin depleted nuclei using an in vitro DNA replication system. Using a murine 3T3 fibroblast model, chemical and genetic inhibition of CDK activity was used to evaluate the role of specific cyclin complexes in post-quiescent cells. CDK inhibition reduced levels of CDC6, MCM2 and PCNA ...
AbstractDNA damage causes stabilization of p53, leading to G1 arrest through induction of p21cip1. A...
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 2004.Includes bibliographi...
S phase is characterized by the replication of DNA and assembly of chromatin. This requires the synt...
Successful duplication of the genome requires incredible accuracy and precision of key cell cycle re...
Timely ubiquitin-mediated protein degradation is fundamental to cell cycle control, but the precise ...
In order for a cell to successfully complete the cell cycle, the cell must accurately duplicate its ...
Cells initiate DNA replication at thousands of DNA replication origins every cell cycle. Minichromos...
Defects in DNA replication are implicated as early and causal events in malignancy. However, the imm...
The mammalian cell cycle is a temporally controlled and intricately regulated process that governs c...
Each and every time a cell divides it must replicate millions of bases of DNA. In order to carry out...
The progression of the cell cycle is a tightly regulated process, regulated by the activities of cyc...
The ultimate aim of the cell cycle is to create an identical daughter cell. Therefore, correct progr...
AbstractMammalian fibroblasts require mitogens in order to exit from G0 (quiescence) and progress th...
AbstractEukaryotic genomes are replicated from large numbers of replication origins distributed on m...
SummaryCyclin-dependent kinases (CDKs) restrict DNA replication origin firing to once per cell cycle...
AbstractDNA damage causes stabilization of p53, leading to G1 arrest through induction of p21cip1. A...
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 2004.Includes bibliographi...
S phase is characterized by the replication of DNA and assembly of chromatin. This requires the synt...
Successful duplication of the genome requires incredible accuracy and precision of key cell cycle re...
Timely ubiquitin-mediated protein degradation is fundamental to cell cycle control, but the precise ...
In order for a cell to successfully complete the cell cycle, the cell must accurately duplicate its ...
Cells initiate DNA replication at thousands of DNA replication origins every cell cycle. Minichromos...
Defects in DNA replication are implicated as early and causal events in malignancy. However, the imm...
The mammalian cell cycle is a temporally controlled and intricately regulated process that governs c...
Each and every time a cell divides it must replicate millions of bases of DNA. In order to carry out...
The progression of the cell cycle is a tightly regulated process, regulated by the activities of cyc...
The ultimate aim of the cell cycle is to create an identical daughter cell. Therefore, correct progr...
AbstractMammalian fibroblasts require mitogens in order to exit from G0 (quiescence) and progress th...
AbstractEukaryotic genomes are replicated from large numbers of replication origins distributed on m...
SummaryCyclin-dependent kinases (CDKs) restrict DNA replication origin firing to once per cell cycle...
AbstractDNA damage causes stabilization of p53, leading to G1 arrest through induction of p21cip1. A...
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 2004.Includes bibliographi...
S phase is characterized by the replication of DNA and assembly of chromatin. This requires the synt...