Time-resolved generation of membrane potential by ba3 cytochrome c oxidase from Thermus thermophilus coupled to single electron injection into the O and OH states

peer-reviewedTwo electrogenic phases with characteristic times of ~ 14 μs and ~ 290 μs are resolved in the kinetics of membrane potential generation coupled to single-electron reduction of the oxidized “relaxed” O state of ba3 oxidase from T. thermophilus (O → E transition). The rapid phase reflects electron redistribution between CuA and heme b. The slow phase includes electron redistribution from both CuA and heme b to heme a3, and electrogenic proton transfer coupled to reduction of heme a3. The distance of proton translocation corresponds to uptake of a proton from the inner water phase into the binuclear center where heme a3 is reduced, but there is no proton pumping and no reduction of CuB. Single-electron reduction of the oxidized “unrelaxed” state (OH → EH transition) is accompanied by electrogenic reduction of the heme b/heme a3 pair by CuA in a “fast” phase (~ 22 μs) and transfer of protons in “middle” and “slow” electrogenic phases (~ 0.185 ms and ~ 0.78 ms) coupled to electron redistribution from the heme b/heme a3 pair to the CuB site. The “middle” and “slow” electrogenic phases seem to be associated with transfer of protons to the proton-loading site (PLS) of the proton pump, but when all injected electrons reach CuB the electronic charge appears to be compensated by back-leakage of the protons from the PLS into the binuclear site. Thus proton pumping occurs only to the extent of ~ 0.1 H+/e−, probably due to the formed membrane potential in the experiment.ACCEPTEDpeer-reviewe