LPS promotes Th2 dependent sensitisation leading to anaphylaxis in a Pru p 3 mouse model
- Rojo, Javier
- Rodriguez, María J.
- Aranda, Ana
- Fernández, Tahia D.
- Cubells-Baeza, Nuria
- Torres, María J.
- Gomez, Francisca
- Palomares, Francisca
- Perkins, James R.
- Diaz-Perales, Araceli
DOIAbstract
Pru p 3 is the major peach allergen in the Mediterranean area. It frequently elicits severe reactions, limiting its study in humans, raising the need for animal models to investigate the immunological mechanisms involved. However, no anaphylaxis model exists for Pru p 3. We aimed to develop a model of peach anaphylaxis by sensitising mice with Pru p 3 in combination with lipopolysaccharide (LPS) as an adjuvant. Four groups of mice were sensitised intranasally: untreated; treated with Pru p 3; treated with LPS; treated with Pru p 3 + LPS. After sensitisation mice were intraperitoneally challenged with Pru p 3 and in vivo and in vitro parameters were evaluated. Only mice in the Pru p 3 + LPS group showed anaphylaxis symptoms, including a decr...
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