Ionizing radiation (IR) produces direct two-ended DNA double-strand breaks (DSBs) primarily repaired by non-homologous end joining (NHEJ). It is, however, well established that homologous recombination (HR) is induced and required for repair of a subset of DSBs formed following IR. Here, we find that HR induced by IR is drastically reduced when post-DNA damage replication is inhibited in mammalian cells. Both IR-induced RAD51 foci and HR events in the hprt gene are reduced in the presence of replication polymerase inhibitor aphidicolin (APH). Interestingly, we also detect reduced IR-induced toxicity in HR deficient cells when inhibiting post-DNA damage replication. When studying DSB formation following IR exposure, we find that apart from t...
The amazing feature of ionising radiation (IR) as a DNA damaging agent is the range of lesions it in...
When cells are exposed to radiation, cellular lesions are induced in the DNA including double strand...
AbstractDNA double strand breaks and blocked or collapsed DNA replication forks are potentially geno...
Ionizing radiation (IR) produces direct two-ended DNA double-strand breaks (DSBs) primarily repaired...
DNA double-strand breaks (DSBs) represent the most biologically significant lesions induced by ioniz...
This study investigated the efficiency of Non-Homologous End Joining (NHEJ) and Homologous Recombina...
Cells have evolved multiple mechanisms to preserve genome integrity and restore structural and funct...
<div><p>The choice of DNA double strand break (DSB) repair pathway is determined at the stage of DSB...
The choice of DNA double strand break (DSB) repair pathway is determined at the stage of DSB end res...
DNA double strand breaks (DSBs) are potential lethal lesions but can also lead to chromosome rearran...
Homologous recombination (HR) and nonhomologous end-joining (NHEJ) are mechanistically distinct DNA ...
DNA double strand breaks (DSBs) are highly cytotoxic lesions that are generated by ionizing radiatio...
Ionising radiation- (IR-) induced DNA double-strand breaks (DSBs) are considered to be the deleterio...
Homologous recombination (HR) is the major mechanism used to repair double-strand breaks (DSBs) that...
Homologous recombination (HR) and non‐homologous end joining (NHEJ) represent distinct pathways for ...
The amazing feature of ionising radiation (IR) as a DNA damaging agent is the range of lesions it in...
When cells are exposed to radiation, cellular lesions are induced in the DNA including double strand...
AbstractDNA double strand breaks and blocked or collapsed DNA replication forks are potentially geno...
Ionizing radiation (IR) produces direct two-ended DNA double-strand breaks (DSBs) primarily repaired...
DNA double-strand breaks (DSBs) represent the most biologically significant lesions induced by ioniz...
This study investigated the efficiency of Non-Homologous End Joining (NHEJ) and Homologous Recombina...
Cells have evolved multiple mechanisms to preserve genome integrity and restore structural and funct...
<div><p>The choice of DNA double strand break (DSB) repair pathway is determined at the stage of DSB...
The choice of DNA double strand break (DSB) repair pathway is determined at the stage of DSB end res...
DNA double strand breaks (DSBs) are potential lethal lesions but can also lead to chromosome rearran...
Homologous recombination (HR) and nonhomologous end-joining (NHEJ) are mechanistically distinct DNA ...
DNA double strand breaks (DSBs) are highly cytotoxic lesions that are generated by ionizing radiatio...
Ionising radiation- (IR-) induced DNA double-strand breaks (DSBs) are considered to be the deleterio...
Homologous recombination (HR) is the major mechanism used to repair double-strand breaks (DSBs) that...
Homologous recombination (HR) and non‐homologous end joining (NHEJ) represent distinct pathways for ...
The amazing feature of ionising radiation (IR) as a DNA damaging agent is the range of lesions it in...
When cells are exposed to radiation, cellular lesions are induced in the DNA including double strand...
AbstractDNA double strand breaks and blocked or collapsed DNA replication forks are potentially geno...