THE INFLUENCE OF CHLORINE ON THE BACTERIUM OF PSEUDOMONAS AERUGINOSA IN WATER

Zadatak ovog završnog rada iz kolegija Mikrobiologija hrane je bio pronaći bakteriju Pseudomonas aeruginosa u otpadnoj komunalnoj vodi. Praktični dio ispitivanja pronalaska bakterije i dezinfekcija vode provedeni su u ovlaštenom laboratoriju Vodovod d.o.o. Slavonski brod na Vodocrpilištu Jelas. Ispitivanje se provodilo na uzorcima vode koji su doneseni u cilju pronalaska bakterije Pseudomonas aeruginosa. U teoretskom dijelu završnog rada opisana je povijest i podjela roda Pseudomonas, kao i bakterije Pseudomonas aeruginosa. Ukratko su navedene značajke bakterije, kao što su morfologija, biokemijske osobine, otpornost, toksičnost, epidemiologija, patogenost za ljude i životinje, kolonizacija i dijagnoza. Kada je izvršen zadatak pronalaska bakterije postupkom membranske filtracije u uzorku otpadne vode, idući zadatak bio je pronaći koncentraciju klora koja će potpuno uništiti bakteriju Pseudomonas aeruginosa, to jest napraviti dezinfekciju vode u kojoj je pronađena ta bakterija. Nakon što je pronađen uzorak vode u kojem se nalazi bakterija koja se tražila napravljena je dezinfekcija klorom u ovisnosti o vremenu. Dezinfekcija klorom je prekidana s otopinom natrij tiosulfat Na2S2O3 u vremenskim intervalima koji su prethodno zadani. Uzorci vode od 100 ml filtrirani su postupkom membranske filtracije s vakuum pumpom, kroz membranski filter papir od 0,45 μm. Sve je provedeno u sterilnim uvjetima kao što je opisano detaljno u radu. Nakon inkubacije od 48 h na 37 °C zapisani su rezultati koji su navedeni u radu.The task of this final work from the Food Microbiology course was to find the bacterium of Pseudomonas aeruginosa in wastewater. The practical part of the bacterial finding and disinfection of water was carried out at a licensed laboratory of Vodovod d.o.o. Slavonski brod at the watercourse Jelas. The experiment was conducted on water samples taken to find the bacterium Pseudomonas aeruginosa. In the theoretical part of the final work, we described the history and classification of Pseudomonas genus, as well as the Pseudomonas aeruginosa bacteria. Briefly, we have outlined the features of the bacterium, such as morphology, biochemical properties, resistance, toxicity, epidemiology, pathogenesis for humans and animals, colonization and diagnosis. When the task of finding a bacterium by membrane filtration in a sample of wastewater was done, the next task was to find a chlorine concentration that would completely destroy the bacterium Pseudomonas aeruginosa. That is to make the disinfection of water in which we found that bacterium. Once the sample of bacterial water is found, disinfection with chlorine was made depending on the time. Chlorine disinfection was interrupted with Na2S2O3 sodium thiosulfate in the time intervals which are previously set. 100 ml water samples were filtered through vacuum with procedure of membrane filtration through a 0,45 μm membrane filter paper. Everything was done under sterile conditions as described in detail in the work. After incubation for 48 h at 37 °C, the results given where given on the paper