Type I pullulanases are enzymes that specifically hydrolyse α-1,6 linkages in polysaccharides. This study reports the analyses of a novel type I pullulanase (PulASK) from Anoxybacillus sp. SK3-4. Purified PulASK (molecular mass of 80 kDa) was stable at pH 5.0–6.0 and was most active at pH 6.0. The optimum temperature for PulASK was 60 °C, and the enzyme was reasonably stable at this temperature. Pullulan was the preferred substrate for PulASK, with 89.90 % adsorbance efficiency (various other starches, 56.26–72.93 % efficiency). Similar to other type I pullulanases, maltotriose was formed on digestion of pullulan by PulASK. PulASK also reacted with β-limit dextrin, a sugar rich in short branches, and formed maltotriose, maltotetraose...
Pullulanase producing Anoxybacillus rupiensis strain NP2 (NCBI accession number: MF355418) was isola...
Thermoanaerobium Tok6-B1 pullulanase (EC 3.2.1.41) was active on alpha 1-6-glucosidic linkages of pu...
A pullulanase I gene from Bacteroides thetaiotaomicron has been cloned. To determine whether the clo...
Type I pullulanase from Anoxybacillus sp. SK3-4 (PulASK) is an unusual debranching enzyme that speci...
An amylopullulanase of the thermophilic Anoxybacillus sp. SK3-4 (ApuASK) was purified to homogeneity...
Enzymes that specifically break down the branch points – debranching enzymes – have been known for m...
Starch and pullulan degrading enzymes are essential industrial biocatalysts. Pullulan-degrading enzy...
Bacillus circulans F-2 amylase-pullulanase enzyme (APE) displayed dual activity with respect to glyc...
The <i>pulA1</i> gene, encoding a novel thermostable type I pullulanase PulA1 from <i>Bacillus</i> s...
Problem statement: Pullulanase is one of the important enzymes in starch industry. Search for the pu...
The pullulanase (EC 3.2.1.41) of Bacillus acidopullulyticus was purified using anion exchange chroma...
The thermophilic Anoxybacillus sp. SK3-4 was formerly isolated from Sungai Klah hot spring, Malaysia...
Panose is a type of functional sugar with diverse bioactivities. The enzymatic conversion bioprocess...
Copyright © 2012 Siew Ling Hii et al. This is an open access article distributed under the Creative ...
Bacterial pullulanase represents one of th e starch-degrading enzymes that are widely used in the st...
Pullulanase producing Anoxybacillus rupiensis strain NP2 (NCBI accession number: MF355418) was isola...
Thermoanaerobium Tok6-B1 pullulanase (EC 3.2.1.41) was active on alpha 1-6-glucosidic linkages of pu...
A pullulanase I gene from Bacteroides thetaiotaomicron has been cloned. To determine whether the clo...
Type I pullulanase from Anoxybacillus sp. SK3-4 (PulASK) is an unusual debranching enzyme that speci...
An amylopullulanase of the thermophilic Anoxybacillus sp. SK3-4 (ApuASK) was purified to homogeneity...
Enzymes that specifically break down the branch points – debranching enzymes – have been known for m...
Starch and pullulan degrading enzymes are essential industrial biocatalysts. Pullulan-degrading enzy...
Bacillus circulans F-2 amylase-pullulanase enzyme (APE) displayed dual activity with respect to glyc...
The <i>pulA1</i> gene, encoding a novel thermostable type I pullulanase PulA1 from <i>Bacillus</i> s...
Problem statement: Pullulanase is one of the important enzymes in starch industry. Search for the pu...
The pullulanase (EC 3.2.1.41) of Bacillus acidopullulyticus was purified using anion exchange chroma...
The thermophilic Anoxybacillus sp. SK3-4 was formerly isolated from Sungai Klah hot spring, Malaysia...
Panose is a type of functional sugar with diverse bioactivities. The enzymatic conversion bioprocess...
Copyright © 2012 Siew Ling Hii et al. This is an open access article distributed under the Creative ...
Bacterial pullulanase represents one of th e starch-degrading enzymes that are widely used in the st...
Pullulanase producing Anoxybacillus rupiensis strain NP2 (NCBI accession number: MF355418) was isola...
Thermoanaerobium Tok6-B1 pullulanase (EC 3.2.1.41) was active on alpha 1-6-glucosidic linkages of pu...
A pullulanase I gene from Bacteroides thetaiotaomicron has been cloned. To determine whether the clo...