Cytological and embryological studies on apospory in Bothriochloa ischaemum L.

Cytological and embryological studies on apomictic species Bothriochloa ischaemum L. were carried out. Our studies revealed that the chromosome number of its root apical cells was 40, indicating that it was a tetraploid cytotype. During the stage of microsporogenesis, meiosis seemed irregular, as the pairing chromosome number of microspore mother cell was more than 20. It was often found that some chromosomes did not assemble in the equatorial plane or moved to the two poles of the cell, a few laggards were seen. Multiporate pollens (22.3%) were often observed. The studies showed that a high frequency (87.8%) of 1–3 or more aposporous embryo sacs developed in one ovule of the species. The mature aposporous sac was usually characterized by an egg cell and one polar nucleus. The egg cell could develop spontaneously into a large proembryo (100–200 μm) mass prior to anthesis. When several aposporous sacs occurred in the same ovule, usually 2 aposporous sacs were involved in pseudogamy and developed into separate endosperm masses in the same ovary. In the low frequency of mature seed, 13.5% twinembryo seedlings could be obtained after mature seeds germinated