Amplified Fragment Length Polymorphism (AFLP) is useful for finding markers associated with QTL for architectural trait in Hedysarum coronarium L.

Amplified fragment length polymorphism (AFLP) has been proposed as a valuable tool for finding mol- ecular markers linked to QTL controlling architectural trait. Segregation of polymorphic AFLP frag- ments was followed in F 2 offsprings Hedysarum coronarium derived from two native wild accesions crossing: Jebel Zit × El Haouaria, which had previously characterised by morphological and AFLP mark- ers. A comparison of profiles from the morphologically contrasted parental individuals and F 2 offspring has been assessed using five AFLP primer combinations. These generated 178 bands revealed by silver- stained denaturing polyacrylamide gels, of which 150 were polymorphic. Several AFLP markers appeared to be implied in the orthotropic form that can assist the selection and improve Hedysarum for- age crop