Correlation Protein and Amino Acid Content in Feed Ingredients with Zinc Binding Protein

. The aim of this study was to assess the correlation between protein content (N) and the amino acid of the feed material to the holding capacity of zinc and to find out the type of amino acids that contribute to bind Zn. Nineteen feedstuffs used in the experiment, namely soybean meal, catton seed meal, coconut meal, palm meal, distillers dried grains with solubles (DDGS), soy sauce waste, tofu waste, blood meal, feathers meal, fish meal, poultry meat meal (PMM) and meat bone meal (MBM), shrimp head meal, cassava leaf flour, sesbania leaf flour, glandiflora leaf flour, leucaenia leaf flour, gliricidia leaf flour, calliandra leaf flour, paraserianthes leaf flour were used. Nitrogen content of all feed ingridient was analyzed using Kejhdahl. The material was immersed in a solution of ZnO with a ratio N: Zn = 10: 1 for 24 hours then dried, subsequently the samples were analyzed to Zn bound in the protein feedstuffs (Zn-proteinate) and amino acid levels. Regression analysis was conducted to determine the correlation between the protein and amino acid content of feed ingredients with Zn-proteinate generated. Results of the study found that the average efficiency of incorporation of Zn-proteinate amounted to 47.87%. Feed ingredients that have the highest level of incorporation of soybean meal (73.71%). There is a positive correlation between crude protein content with the level of incorporation of Zn (Zn-proteinate). The results of non-linear regression analysis of the amino acid to the Zn-proteinate indicate that there were four amino acids that have a positive correlation with Zn-proteinate that aspartic acid, glutamic acid, arginine and tyrosine. The conclusion of this study is to increase the protein content of feed ingredients and amino acids aspartic acid, glutamic acid, arginine and tyrosine lead to increased levels of Zn-proteinate (Zn incorporation)