Pengaruh Formalin Peroral Dosis Bertingkat Selama 12 Minggu Terhadap Gambaran Histopatologis Gaster Tikus Wistar

Background: formalin is a substance generally used for human body preservation, as well as the industrial and pathology anatomy examination substance. Formalin is currently applied as food preservation, despite its danger for health. Based on the investigation and laboratory test conducted by Balai Besar Pengawasan Obat dan Makanan (BPOM) in Jakarta, there were some products found with formalin addition such as salted fish, noodle, and tofu.Aim: To prove the effect of gradually per oral dosage of formalin to the change of histopathological image of wistar rat gaster.Method: This research was conducted using laboratory experiment with post test only control group design. There were 20 wistar rats which had fulfilled the inclusion and exclusion criteria and then adapted for seven days. After completed the adaptation period, the samples were divided by simple random sampling into 4 groups. K is control group without per oral formalin dosage. P1, P2, and P3 are case groups with per oral formalin dosage of 50mg/kgW/day, 100mg/kgW/day, and 200mg/kgW/day respectively. After 12 weeks, all samples' gasters were taken and they underwent histopathologic examination. Data were described on table, images and computer program.Results: The highest rate of gastric cell damage is on group P3. By Kruskal Wallis test there was a significant difference (p=0.000). Post Man Whitney Test found significant differences in K-P1 (p=0,008), K-P2 (p=0,008), K-P3 (p=0,008), P1-P2 (p=0,010), P1-P3 (p=0,008), whereas on P2-P3 there was no significant different found (p=0,456).Conclusion: Gradual per oral formalin dose for 12 weeks caused histopathological changes on wistar rat's gaster. The changes showed including epithelial desquamation, epithelial erosion and epithelial ulceration