Characteristic of Frozen-thawed Epididymal Spermatozoa and Refrigerated Storage of Ram Spermatozoa

Post-mortem spermatozoa recovery is an important technique for obtaining germplasm reservesfrom genetically valuable animals or endangered species. The purpose of this study was to studywhether ram spermatozoa within epididymides stored at 4º C for 24 and 48 h remain their motility andviability. The characteristic of ram epididymal spermatozoa after freezing and thawing was alsoobserved. Six pairs of ram testes with attached epididymides were used in this study. The motility ofcontrol spermatozoa was well maintained throughout the dilution procedure (83.3±1.1, 80±1.3, and80±1.3% for collection, Niwa and Sasaki freezing -1 extender (NSF-1 and NSF-2 groups respectively);but declined (P<0.05) after freezing and thawing (38.3±3.1%). Motile and viable spermatozoa could berecovered from epididymides up to 48 h of storage, although their quality declined significantly(P