Impact of the gold support on the electrocatalytic oxidation of sugars at enzyme-modified electrodes

The influence of gold support on the bioelectrocatalytic activity of glucose oxidase and cellobiose dehydrogenase immobilized on self-assembled monolayer-modified high surface area gold electrodes such as rough gold and gold nanoparticles has been studied. The two types of enzyme-modified electrodes showed very high activity towards sugar oxidation. However, it has been shown that the largest part of this electrocatalytic activity comes from the underlying gold surface. These findings are of special importance for bioelectrochemical studies of enzymatic electrodes, where the immobilization support might show electrocatalytic properties toward the substrates of investigated enzymes