Rosella (Hibiscus sabdariffa,l)extract increase the macrophage phagocytic activity in dmba (7,12-dimetilbenz(α)antrasen)induced sprague dawley rat

The immune system can be modulated with immunogen, including immunogen ferom plants. Over exposure of free radicals were reported to suppress the immune functions.Previous study reported the potency of H.sabdariffa (rosella)extract to increase the phagocytic activity in vitro. The objective of this study was to explore the effect of rosella extract in increasing macrophage phagocytic in DMBa induced rat in vivo. Rosella calyx were extracted with maceration method using 70%ethanol. The animals were induced with DMBA 15 mg/kgBW single dose. After DMBA induce, at the same day,animals were treated with rosella extract with dose 10,50 and 100mg/kgBW for 33 days. After treatment the animals were sacrified and macrophage were isolated form peritoneal fluid. The results showed that phagocytic activity of macrophages showed significant diffrences between the groups with the DMBA and EE dose 10,50 and 100 mgkgBW. Nitruc oxide (NO) secretion of Reactive Oxygen Intermediate (ROI) and expression of IL-12 levels were also found to incease.The total flavonoid content was obtained 2,465% and total polyphenol content was obtained 6,03 g GAE/100 g extract