Detection of Leaf Rust Resistance Genes Lr9 and Lr10 in Wheat (Triticum aestivum) by PCR Based STS Markers

Leaf rust resistance genes Lr9 and Lr10 were detected in wheat (Triticum aestivum) genotypes by PCR based STS markers. Out of sixty-two elite wheat genotypes, screened for the presence of leaf rust resistance gene Lr10, nineteen genotypes revealed the presence through PCR analysis with the primers specific to Lr10 gene. Validation of the marker for Lr9 gene in the parental lines followed by successful detection of the gene in F4 lines out of cross HP1633 (Lr9) X HP1776, was also done. Usefulness of molecular markers for the detection of rust resistance genes in different genotypes is discussed