In this protocol, we describe a procedure for incorporating ATP-binding cassette (ABC) transporters into large unilamellar vesicles (LUVs) and assays to determine ligand binding and solute translocation by these membrane-reconstituted systems. The reconstitution technique as described has been optimized for ABC transporters but can be readily adapted for other types of transport systems. Purified transporters are inserted into detergent-destabilized preformed liposomes and detergent is subsequently removed by adsorption onto polystyrene beads. Next, Mg-ATP or an ATP-regenerating system is incorporated into the vesicle lumen by one or more cycles of freezing-thawing, followed by extrusion through polycarbonate filters to obtain unilamellar v...
AbstractThe oligopeptide transporter Opp is a five-component ABC uptake system. The extracytoplasmic...
AbstractThe transporter associated with antigen processing (TAP) is essential for peptide loading on...
Protein translocation is one of the examples of a complex membrane-bound biological process that has...
In this protocol, we describe a procedure for incorporating ATP-binding cassette (ABC) transporters ...
ATP-binding cassette (ABC) transporters are vital to any living system and are involved in the trans...
ATP‐binding cassette (ABC) transporters are vital to any living system and are involved in the trans...
ATP-binding cassette (ABC) transporters use ATP to drive solute transport across biological membrane...
AbstractATP Binding Cassette (ABC) transporters play prominent roles in numerous cellular processes ...
ATP-binding cassette (ABC) transporters are a large group of membrane protein complexes that couple ...
ABC (ATP-binding-cassette) transporters carry out many vital functions and are involved in numerous ...
The oligopeptide transporter Opp is a five-component ABC uptake system. The extracytoplasmic lipid-a...
The oligopeptide transporter Opp is a five-component ABC uptake system. The extracytoplasmic lipid-a...
AbstractThis paper describes the formation of giant proteoliposomes containing P-glycoprotein (P-gp)...
AbstractThe oligopeptide transporter Opp is a five-component ABC uptake system. The extracytoplasmic...
AbstractThe transporter associated with antigen processing (TAP) is essential for peptide loading on...
Protein translocation is one of the examples of a complex membrane-bound biological process that has...
In this protocol, we describe a procedure for incorporating ATP-binding cassette (ABC) transporters ...
ATP-binding cassette (ABC) transporters are vital to any living system and are involved in the trans...
ATP‐binding cassette (ABC) transporters are vital to any living system and are involved in the trans...
ATP-binding cassette (ABC) transporters use ATP to drive solute transport across biological membrane...
AbstractATP Binding Cassette (ABC) transporters play prominent roles in numerous cellular processes ...
ATP-binding cassette (ABC) transporters are a large group of membrane protein complexes that couple ...
ABC (ATP-binding-cassette) transporters carry out many vital functions and are involved in numerous ...
The oligopeptide transporter Opp is a five-component ABC uptake system. The extracytoplasmic lipid-a...
The oligopeptide transporter Opp is a five-component ABC uptake system. The extracytoplasmic lipid-a...
AbstractThis paper describes the formation of giant proteoliposomes containing P-glycoprotein (P-gp)...
AbstractThe oligopeptide transporter Opp is a five-component ABC uptake system. The extracytoplasmic...
AbstractThe transporter associated with antigen processing (TAP) is essential for peptide loading on...
Protein translocation is one of the examples of a complex membrane-bound biological process that has...