The peroxisomal membrane protein Pex3p of Hansenula polymorpha: analysis of its function in peroxisome biogenesis

In this thesis the methytotrophic yeast Hansenula polymorpha was used as a model organism to study peroxisome biogenesis. H. polymorpha cells are able to grow on methanol as a sole carbon source, which requires the compartmentalization of part of its metabolism in peroxisomes. The specific aim of this study was to investigate the molecular mechanisms underlying the biogenesis of the peroxisomal membrane. In the early 1990's H. polymorpho mutants have been isolated and setected for defects in peroxisome function, primarily by their inabitity to grow on methanol. One of these mutants, which lacked any detectable peroxisomal membrane structures, was studied in detail. ... Zie: Summary