Characterization of a redox active cross-linked complex between cyanobacterial photosystem I and soluble ferredoxin

Lelong, Cécile,
Boekema, Egbert J.,
Kruip, Jochen,
Bottin, Hervé,
Rögner, Matthias,
Sétif, Pierre,

Publication date

January 1996

Abstract

A covalent stoichiometric complex between photosystem I (PSI) and ferredoxin from the cyanobacterium Synechocystis sp. PCC 6803 was generated by chemical cross-linking. The photoreduction of ferredoxin, studied by laser flash absorption spectroscopy between 460 and 600 nm, is a fast process in 60% of the covalent complexes, which exhibit spectral and kinetic properties very similar to those observed with the free partners. Two major phases with t1/2 <1 µs and ≈10-14 µs are observed at two different pH values (5.8 and 8.0). The remaining complexes do not undergo fast ferredoxin reduction and 20-25% of the complexes are still able to reduce free ferredoxin or flavodoxin efficiently, thus indicating that ferredoxin is not bound properly in thi...

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Characterization of a redox active cross-linked complex between cyanobacterial photosystem I and soluble ferredoxin

Abstract

Extracted data

Characterization of a redox active cross-linked complex between cyanobacterial photosystem I and soluble ferredoxin

Abstract

Extracted data

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