A Bacillus megaterium Plasmid System for the Production, Export, and One-Step Purification of Affinity-Tagged Heterologous Levansucrase from Growth Medium
- Malten, Marco,
- Biedendieck, Rebekka,
- Gamer, Martin,
- Drews, Ann-Christin,
- Stammen, Simon,
- Buchholz, Klaus,
- Dijkhuizen, Lubbert,
- Jahn, Dieter,
DOIAbstract
A multiple vector system for the production and export of recombinant affinity-tagged proteins in Bacillus megaterium was developed. Up to 1 mg/liter of a His6-tagged or Strep-tagged Lactobacillus reuteri levansucrase was directed into the growth medium, using the B. megaterium esterase LipA signal peptide, and recovered by one-step affinity chromatography.
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