Bleomycin resistance: a new dominant selectable marker for plant cell transformation
- Hille, Jacques,
- Verheggen, Frank,
- Roelvink, Peter,
- Franssen, Henk,
- Kammen, Ab van,
- Zabel, Pim,
DOIAbstract
Plant cells are sensitive to the antibiotic bleomycin, a DNA damaging glycopeptide. A bleomycin resistance determinant, located on transposon Tn5 and functional in bacteria, has been cloned in a plant expression vector and introduced into Nicotiana plumbaginifolia using Agrobacterium tumefaciens. The expression of this determinant in plant cells confers resistance to bleomycin and allows selection of transformed plant cells.
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