Grape seed extract reduces the severity of selected disease markers in the proximal colon of dextran sulphate sodium-induced colitis in rats

BackgroundGrape seed extract (GSE) constitutes a rich source of procyanidins. GSE has been demonstrated to exert encouraging anti-inflammatory and anti-ulcer properties in experimental settings, although its effects on inflammation of the colon remain undefined.AimTo determine the effects of GSE in a rat model of dextran sulphate sodium (DSS) for ulcerative colitis.MethodsMale Sprague-Dawley rats were gavaged daily (days 0-10) with GSE (400 mg/kg). Ulcerative colitis was induced by substituting DSS (2 % w/v) for drinking water from days 5-10. A sucrose breath test was performed on day 11 to determine small bowel function and intestinal tissues were collected for histological analyses. Statistical analysis was by one-way or repeated-measures ANOVA and p ResultsCompared to DSS-treated controls, GSE significantly decreased ileal villus height (14 %; p ConclusionsGSE decreased the severity of selected markers of DSS-induced colitis in the distal ileum and proximal colon, suggesting the potential as an adjuvant therapy for the treatment of ulcerative colitis. Future studies of GSE should investigate alternative delivery methods and treatment regimens, further seeking to identify the individual bioactive factors.Ker Y. Cheah, Susan E. P. Bastian, Thomas M. V. Acott, Suzanne M. Abimosleh, Kerry A. Lymn, Gordon S. Howart