Toward a full characterization of native starch: separation and detection by size-exclusion chromatography
- Hoang, N.
- Landolfi, A.
- Kravchuk, A.
- Girard, E.
- Peate, J.
- Hernandez, J.
- Gaborieau, M.
- Kravchuk, O.
- Gilbert, R.
- Guillaneuf, Y.
- Castignolles, P.
DOIAbstract
The structure of starch molecules is relevant to nutrition and industrial applications. Size-exclusion chromatography (SEC, also known as GPC) of native starch generally suffers non-satisfactory repeatability and reproducibility of the dissolution and separation. This work combines two polar organic solvents: dimethylsulfoxide for complete dissolution and dimethylacetamide to limit shear degradation. The separation is as repeatable as that of polystyrene standards performing dissolution and separation at 80 degrees C. Successful covalent-labeling on the glucose unit is claimed to be published here for the first time in non-degradative conditions and allows the use of UV detector with significantly higher sensitivity than with a refractomete...
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