A bioprocessing mode for simultaneous fungal biomass protein production and wastewater treatment using an external air-lift bioreactor

A pilot plant investigation for bioprocessing has been undertaken to develop a simple, non-aseptic, low-cost single process for production of fungal biomass protein (FBP) and wastewater treatment using starch processing wastewater. It has been confirmed that the newly developed external air-lift bioreactor was very suitable for bioconversion of starch materials and FBP production by the microfungi Aspergillus oryzae and Rhizopus arrhizus. Bioproduct yields of 8.5 g dm⁻³ of FBP that contained 46-50% protein were obtained within a comparatively short retention time. A fungal biomass productivity in a range of 0.85-0.92 g dm⁻³ h⁻¹ and removals of total suspended solids and 95% COD were achieved in batch, semi-continuous and continuous processes. The operation modes of the semi-continuous and continuous processes demonstrated a high biological dynamics in fungal biomass productivity and COD reduction. The semi-continuous process appeared to be the most practical mode.Bo Jin, Q Yu, J (Hans) van Leeuwe