Novel polyethylene glycol derivative suitable for the preparation of mono-PEGylated protein
- Yun, Q.
- Chen, T.
- Zhang, G.
- Bi, J.
- Ma, G.
- Su, Z.
Publication date
January 2005
DOI Publisher
Springer Science and Business Media LLC ISSN
0141-5492 Journal
Biotechnology Letters Citation count (estimate)
1Abstract
The definitive version may be found at www.springerlink.comA novel methoxypolyethylene glycol (mPEG) derivative, containing a reactive group of 1-methyl pyridinium toluene-4-sulfonate, was synthesized and characterized. The mPEG derivative was successfully conjugated with two proteins: recombinant human granulocyte-colony stimulating factor (rhG-CSF) and consensus interferon (C-IFN). Homogeneous mono-PEGylated proteins were obtained which were identified by high performance size-exclusion chromatography and MALDI-TOF mass spectrometry. The biological activities of the mono-PEGylated rhG-CSF and the mono-PEGylated C-IFN were maintained at 90% and 88%, respectively.Qiang Yun, Ting Chen, Guifeng Zhang, Jingxiu Bi, Guanghui Ma and Zhiguo S
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