Method of amplifying nucleic acid

Patent: WO/2006/094360. PCT/AU2006/00031. Published14.09.2006The present invention provides a method of amplifying nucleic acid using a PCR-based assay. For example, the present invention provides a method for amplifying a nucleic acid in a single closed-tube, the method comprising performing a first round amplification with an amount of a tagged locus-specific primer or set thereof suitable for performing exhaustive PCR. In a second round amplification, the first round amplification product is amplified using tag primers having lower melting temperature than the tagged locus-specific primers and annealed to the incorporated tag sequence in said first round amplification product at a lower annealing temperature than used in the first round. The present invention also provides a multiplex method of amplification. The present method also provides methods of characterising or identifying individuals or for diagnosing a disease or disorder