PHYLOGENETIC ANALYSIS OF \u3ci\u3eMacrophomina phaseolina\u3c/i\u3e (Tassi) Goid. FROM COMMON BEANS AND OTHER HOSTS

The fungus Macrophomina phaseolina (Tassi) Goid. (MP) is the causal agent of charcoal rot disease that affects more than 100 plant families worldwide and causing significant yield losses (Hernández-Delgado et al., 2011). Despite the broad host range, only a single species is currently recognized: M. phaseolina (NCBI, 2017; http://ncbi.nlm.nih,gov). Charcoal rot disease is favored by drought and high temperatures stresses. MP symptoms are characterized by dark lesions at seedling stems that after cover the complete plant destroying vascular vessels. Finally, systemic chlorosis, wilting, defoliation and microsclerotia and pycnidia growing on stem epidermis are distinguished (Kaur et al., 2012). Recently, Sarr et al. (2014) reported two species within the genus Macrophomina: M. phaseolina and M. pseudophaseolina supported by slight differences on conidia morphology and phylogenetic analysis based on five loci on MP isolates from Senegal and other countries. Our results support that even M. phaseolina shows a broad genetic variability due host and geographical origin variations, no species or subspecies are evident (Reyes-Franco et al., 2006). The aim of this work was to analyze MP isolates from different host and geographical origins based on four genetic markers and to determine their phylogenetic relationships