Human mesenchymal stem cells (hMSCs) are one of the most widely researched stem cell types with broad applications from basic research to therapeutics, the majority of which require introduction of exogenous DNA. However, safety and scalability issues hinder viral delivery, while poor efficiency hinders nonviral gene delivery, particularly to hMSCs. Here, we present the use of a pharmacologic agent (glucocorticoid) to overcome barriers to hMSC DNA transfer to enhance transfection using three common nonviral vectors. Glucocorticoid priming significantly enhances transfection in hMSCs, demonstrated by a 3-fold increase in efficiency, 4–15-fold increase in transgene expression, and prolonged transgene expression when compared to transfection w...
AbstractDNA uptake can be facilitated by addition of physiological amounts of 11β-hydroxy glucocorti...
Abstract Background Mesenchymal stem cells (MSCs) are multipotent stem cells that can be isolated an...
In recent decades, human mesenchymal stem cells (hMSCs) have gained momentum in the field of cell th...
Human mesenchymal stem cells (hMSCs) are one of the most widely researched stem cell types with broa...
Safe and efficient gene delivery to human mesenchymal stem cells (hMSCs) is vital for their clinical...
Background: Because of unique roles in wound healing, trophic tissue support, immunomodulation, diff...
Human mesenchymal stem cells (hMSCs) are under study for cell and gene therapeutics because of their...
Human Mesenchymal Stem Cells: Human mesenchymal stem cells (hMSCs) are a multipotent cell, meaning t...
Human mesenchymal stem cells (hMSCs) are under intense study for applications of cell and gene thera...
Human mesenchymal stem cells (hMSCs) are primary cells with high clinical relevance that could be en...
Background: Human mesenchymal stem cells (hMSCs) are intensely researched for applications in cell t...
Human mesenchymal stem cells (hMSCs) are under research for applications in cell-based therapies due...
Human mesenchymal stem cells (hMSCs) are under study for applications in tissue engineering and rege...
Human mesenchymal stem cells (hMSCs) are primary cells with high clinical relevance that could be en...
Background: Mesenchymal stem cells (MSCs) are multipotent stem cells that can be isolated and expand...
AbstractDNA uptake can be facilitated by addition of physiological amounts of 11β-hydroxy glucocorti...
Abstract Background Mesenchymal stem cells (MSCs) are multipotent stem cells that can be isolated an...
In recent decades, human mesenchymal stem cells (hMSCs) have gained momentum in the field of cell th...
Human mesenchymal stem cells (hMSCs) are one of the most widely researched stem cell types with broa...
Safe and efficient gene delivery to human mesenchymal stem cells (hMSCs) is vital for their clinical...
Background: Because of unique roles in wound healing, trophic tissue support, immunomodulation, diff...
Human mesenchymal stem cells (hMSCs) are under study for cell and gene therapeutics because of their...
Human Mesenchymal Stem Cells: Human mesenchymal stem cells (hMSCs) are a multipotent cell, meaning t...
Human mesenchymal stem cells (hMSCs) are under intense study for applications of cell and gene thera...
Human mesenchymal stem cells (hMSCs) are primary cells with high clinical relevance that could be en...
Background: Human mesenchymal stem cells (hMSCs) are intensely researched for applications in cell t...
Human mesenchymal stem cells (hMSCs) are under research for applications in cell-based therapies due...
Human mesenchymal stem cells (hMSCs) are under study for applications in tissue engineering and rege...
Human mesenchymal stem cells (hMSCs) are primary cells with high clinical relevance that could be en...
Background: Mesenchymal stem cells (MSCs) are multipotent stem cells that can be isolated and expand...
AbstractDNA uptake can be facilitated by addition of physiological amounts of 11β-hydroxy glucocorti...
Abstract Background Mesenchymal stem cells (MSCs) are multipotent stem cells that can be isolated an...
In recent decades, human mesenchymal stem cells (hMSCs) have gained momentum in the field of cell th...