Add to ORKGBlood samples were taken from the orbital sinus (venous) of normal (C3H/C(sa)C(sa)), acatalasemic (C3H/C(sb)C(sb)) and hypocatalasemic (acatalasemic heterozygote, C3H/C(sa)C(sb)) mice. Packed red cells prepared from mice were washed three times with cold 0.9% NaCl solution to remove the buffy coat. One volume of red cells was hemolyzed with 1.5 volumes of distilled water. The various mouse hemolysates were fractionated into A, B and C fractions by DEAE cellulose column chromatography with a discontinous buffer system. Catalase activity in the eluate was determined by the perborate method, and the eluate was concentrated to 1.0PU/ml. Agarose isoelectric focusing was performed in a pH 3-to-10 Pharmalyte gradient gel at 81℃. The distribution o...