Studies on lymphadenopathy by tissue culture Part III Effects of antileukemic agents on the lymph node tissue culture of murine lymphatic leukemia

The effects of several antileukemic agents on the function of leukemic lymph nodes were studied with the clinical tissue culture of lymph nodes of murine lymphatic leukemia. First the author performed clinical tissue culture of lymph nodes from a normal mouse with adition of urethane, alkylating agents, 6-mercaptopurine and steroid hormones in serial concentrations and established their maximal concentration in the medium, which did not inhibit the lymph node cell growth. Then, the author observed the effects of direct adition of urethane, alkylating agents, 6-mercaptopurine and steroid hormones at the maximal concentration on the relative growth of the tissue, wandering velocity of the cells and cell density in the lymph node tissue culture of murine lymphatic leukemia, and obtained the following results. In acute lymphatic leukemia the inhibitory effects could be recognized in the descending order of prednisolone, cortisone and ACTH; and nitromin showed a slight inhibition, but the other antileukemic agents exerted little inhibitory action