Studies on Donaggio Reaction in Anderson's Urinary Mucoprotein and Changes in the Excretion of Anderson's Mucoprotein in Fatigue Condition

The author performed Donaggio reaction and Akamatsu-Shinmei reaction usiug urinary mucoprotein A(1), A(2), A(3), isolated by the benzoic acid adsorption method of Anderson, Tamm's urinary mucoprotein, urinary mucoprotein obtained by talcum powder adsorption method and Winzler's serum mucoprotein. The author also determined the excretion of Anderson's mucoprotein in fatigue condition. The results obtained are as follows. 1) Anderson's mucoprotein proves to be strongly positive to Donaggio reaction and the concentration showing one Donaggio unit is 50-100γ/ml, showing the activity almost similar to that of the Donaggio reaction-positive substance performed by talcum powder adsorption method. 2) The activity of Donaggio reaction of the human serum mucoprotein processed by Winzler's method, whose concentration showing one Donaggio unit being around 31.2γ/ml, seems to be higher than that of the Donaggio reaction-positive substance by talcum powder adsorption method. 3) Anderson's urinary mucoprotein is also positive to Akamatsu-Shinmei reaction. 4) Tamm's urinary mucoprotein is Donaggio reaction negative, but after heating it becomes weakly positive, while to Shinmei reaction, it is weakly positive and heating increases its positivity still further. 5) The concentration of urinary mucoprotein prepared by Anderson's quantitative method, increased in the workers of a department store after the work, and in soccer players after the game. Moreover, the quantity of hourly excretion of urinary mucoprotein also increased after the work in the case of workers in the department store. 6) Donaggio reaction values and the quantities of Anderson's urinary mucoprotein fluctuated in parallel to each other after the work and after the game. 7) The recovery rate of the Donaggio reaction values determined by benzoic acid adsorption method of Anderson proved to be 30-50 per cent showing the values close to those by talcum powder adsorption method of Honda. 8) From these results, it is deduced that the mucoprotein (Donaggio reaction positive substance) prepared by the talcum powder adsorption method resembles very closely to Anderson's mucoprotein and these two are most likely derived from serum α(1)-globulin, and Tamm's mucoprotein that is negative to Donaggio reaction is in all probability a different mucoprotein excreted in the urinary tract