Studies on the Isortion of Iron in the HbO(2)-KCN-H(2)O(2) Reaction System in Vitro Part III. Studies on So-called Easy Split Iron (G. Barkan) in the Hb-KCN-H(2)O(2) Reaction system

The Hb-KCN-H(2)O(2) reaction system was observed on the presence of nitrogen gas and CO gas, since the vicissitude of easy aplit iron. And the results were as follows. 1. Investigating this reaction system in the presence of nitrogen gas, the increase of easy spilt iron stopped for 30 minutes, but its yielded dosis was twice at 60 minutes after the aeration of oxygen. 2. Acting only KCN and H(2)O(2) in the HbO-KCN-H(2)O(2) reaction system under the nonoxygen condition, it reached up to the highest fixed value at 20 miuutes and the reaction did not show any progress on the aeration of oxygen after 20 minutes. 3. Acting KCN and H(2)O(2) to reduced hemoglobin, the increase of yielded dosis was abruptly seen around 5 minutes and it progressed slowly untill 60 minnutes, but the yielded dosis became 1.4 times on the aeration of oxygen at that time. 4. The slowness or quickness of reaction was observed on the action of KCN after the action of Hb and H(2)O(2) in the presence of nitrogen gas and on the action of H(2)O(2) after the action of Hb and KCN in the presence of nitrogen gas, but the yielded dosis was both 1.5 times after the action of molecular oxygen to them. 5. The Hb-KCN-H(2)O(2) reaction system was progressed in the presence of nitrogen gas, CO gas was acted to the reaction solutlon in each time and then the yielded dosis of easy split iron was observed, and the reaction stopped for 30 minutes, but the yielded dosis became twice on the aeration of oxygen to it. 6. The progress of the above reaction system could be explained without discrepancy by the concept of E. E' by G. Barkan and the condition of them at the isolated time in the presence of nitrogen gas or CO gas. 7. This reaction system progressed from Hb or HbO(2) and the direct progress from COHb could not be existed