Morphological Studies on Human Ascites Tumor Cells by Tissue Culture Part 3. Influences of anticancerogenic agents on human ascites tumor cells

By performing the tissue culture of cell suspension similarly as in Part 2, using the cells in ascites of the patients with coelothelioma but loading five anticancerogenic agents such as colcemide, carzinophilin, glucosamine, nitromine and unsaturated fatty acid extract of roentgenized liver (Yamamoto) the author carried out a series of observations on the morphological changes of the cells under culture and also compared the anticancerogenic mechanism of each of these drugs. The following are the results. 1) In the case loaded with colcemide a marked degeneration of cytoplasma and the abnormality in cell division can be recognized. 2) In the case loaded with carzinophilin the pyknosis and disintegration of the nucleus and cytoplasma can be observed. 3) In the case loaded with glucosamine the same as in (2), the pyknosis and disintegration of the nucleus and cytoplasma can be seen. 4) In the case of nitromine, the swelling and destruction of the nucleus and cytoplasma as well as the formation of bubbles can be recognized. 5) In the case loaded with unsaturated fatty acid extract of the roentgenized liver, the pyknosis and disintegration of the nucleus and the disintegration and swelling of cytoplasma, namely, the mechanism acting in two ways as carzinophilin and nitromine combined, can be recognized. As can be seen from above, it has been confirmed that the anticancerogenic mechanism of each of these drugs is characteristic respectively