Studies on the Metabolism of Bile Pigment in Vivo Part 1 Studies on the Transformation of Indirect Bilirubin to Direct in vivo

The transformation of indirect belirubin to direct was observed on the action of various organ's tissues to the serum solution of the indirect bilirubin prepared from the jaundiced urine. The results are as follows. 1. The indirect bilirubin prepared from the jaundiced urine is partly transformed to direct bilirubin on the preparation of its serum solution. 2. The action transforming indirect bilirubin to direct bilirubin is observed by the use of the liver and lung tissues of healthy rabbit. The possibility of the above action is also expected by the use of the cerebllum and skin, but the action can not be obtained by the use of the heart, kidney, adrenal gland, medulla oblongata, cerebllum and peritoneum. 3. The function of the reticuloendothelial system seems not to participate in the transformation of indirect bilirubin to direct, since the results observed by the use of the organ's tissues in the rabbit with thrombosis of the reticuloendothelial system are as same as the results observed by the use as healthy rabbit. 4. The action transforming indirect bilirubin to direct bilirubin is obstructed by the use of the liver tissue in the rabbit with administration of carbon tetrachloride, but not by the use of their lung tissues. Carbon tetrachloride, therefore, seems not to influence on the lung. 6. The reduction of biliberdin in rabbit bile to bilirubin is stimulated in different degree, except on the use of sodium citrate, on the action of the liver tissue to the treated rabbit bile by adding of glucose, sodium lactate, alchol, sodium succinate and sodium citrate etc